Journal: Communications Biology

Article Title: Advancing peristalsis deciphering in mouse small intestine by multi-parameter tracking

doi: 10.1038/s42003-023-05631-2

Figure Lengend Snippet: The experimental setup for studying peristalsis: a Lateral view, b Front view. PLUGSYS Transducer Amplifier Modules-A (TAM-A, Harvard apparatus, USA), CVX400 series vision system with multi-spectrum LumiTrax light (Keyence, USA) and infrared-LED sensor drop counters with LabQuest Mini interface (Vernier instruments, USA) are used to measure intraluminal pressure, diameter changes, longitudinal movements, and fluid output in intestinal segments. c Representative image showing jejunal segment mounted in tissue perfusion bath. Jejunal segment with 4 digital edge width ( EW ) trackers and one PatternTrax placed along the length of the intestine. EW trackers track the diameter change with time. PatternTrax measures the longitudinal movement with time using surface characteristics on the intestine such as blood vessels. d The luminal perfusate passing through the intestinal segments were made to fall through a specified area of the drop-counter so that the LED light falling on the detector is blocked, resulting in the generation of a digital signal that is then captured by a data collection interface. The data are converted into volume and expressed in ml/min by the data acquisition program. e Intestinal segments were perfused in water-jacketed tissue bath (Mayflower, USA). The oral and aboral end of the tissues are connected to the pressure transducers and the pressure recordings obtained were amplified using TAM-A.

Article Snippet: PLUGSYS Transducer Amplifier Modules-A (TAM-A, Harvard apparatus, USA), CVX400 series vision system with multi-spectrum LumiTrax light (Keyence, USA) and infrared-LED sensor drop counters with LabQuest Mini interface (Vernier instruments, USA) are used to measure intraluminal pressure, diameter changes, longitudinal movements, and fluid output in intestinal segments. c Representative image showing jejunal segment mounted in tissue perfusion bath.

Techniques: Amplification